edman degradation

N-terminal sequencing by Edman degradation, is a traditional method for sequencing protein, and still has advantages for protein analysis that cannot readily be obtained by other analysis methods. Edman degradation is a cyclic procedure: N-terminal amino acid residues is labeled and cleaved off from the peptides or proteins at a time, and is identified by chromatography. Generally, the N-terminal amino group of the protein is reacted with phenyl isothiocyanate to form a phenylthiocarbamoyl derivative. Under mildly acidic conditions, the derivative is released from the rest of the protein as a cyclic phenylthiohydantoin (PTH) amino acid. The released PTH amino acid is identified by high performance liquid chromatography (HPLC) or HPLC-MS. The remainder of the peptide is intact and available for another round of labeling and release. edman degradation https://www.creative-proteomic....s.com/pronalyse/n-te

pegylation

The identification of protein modification sites and the identification of heterogeneity are the key links in the quality control of modified proteins, especially the analysis of PEGylation sites and identification of PEGylation heterogeneity are related to the pharmacokinetics, pharmacodynamic properties and stability of protein drugs. Therefore, the analysis of PEGylation sites and identification of PEGylation heterogeneity are important biochemical technologies in the research and development of polyethylene glycol modified protein drugs. MALDI-TOF MS and ESI-MS technology methods can be used to analyze of PEGylation sites and identify the PEGylation heterogeneity of drug proteins. pegylation https://www.creative-proteomic....s.com/pronalyse/pegy

flow cytometry

Flow cytometry service can quickly measure a series of important biophysical and biochemical characteristics of cells, such as its ability to identify cell surface proteins to characterize intracellular proteins, detect lymphocyte subtypes (TBNK), blood diseases typing, determination of tumor markers, etc., and provides a variety of different techniques to assess the specific characteristics of adaptability and innate immune function. Flow cytometry can perform high-throughput and powerful quantitative analysis, and provides important technical support for the analysis of cell efficacy in biopharmaceuticals and the need for complex cellular data in biopharmaceutical research and development and clinical trials. flow cytometry https://www.creative-proteomic....s.com/pronalyse/flow

hdx ms

Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful and important tool used to study protein conformation and dynamics, protein-protein interactions, protein-small molecule interactions, and protein-RNA interactions. HDX-MS takes advantage of the naturally proton exchange occurring at amides on proteins, and deuterium can replace the protein backbone hydrogens upon exposure of the protein to a D2O-based buffer. According to the protein's structure, the exchange rates varies. If the hydrogen-bonded segments are tight, the exchange rate is low; the exchange rate is high when the regions are disordered. hdx ms https://www.creative-proteomic....s.com/pronalyse/hydr

brassinosteroid hormone

Creative Proteomics has deep industry and business process expertise, global resources, and a good reputation in providing contract services specialized for metabolomics. brassinosteroid hormone https://metabolomics.creative-....proteomics.com/brass